Render Target: STATIC
Render Timestamp: 2024-11-29T11:42:02.489Z
Commit: cd2fae6ca3f811b1ddb1df24ac291ed56d5d501b
XML generation date: 2024-10-24 19:16:11.212
Product last modified at: 2024-09-11T10:00:09.913Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77

CPS1/Hep Par-1 (OCH1E5) Mouse mAb (Clone previously known as Hepatocyte Specific Antigen) #96422

Filter:
  • IHC

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa)
    Source/Isotype Mouse IgG1
    Application Key:
    • IHC-Immunohistochemistry 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    IHC Leica Bond 1:200 - 1:800
    Immunohistochemistry (Paraffin) 1:100 - 1:400

    Storage

    Supplied in a PBS-based buffer containing sodium azide as a preservative. Stable for 12 months when stored at 4°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    CPS1/Hep Par-1 (OCH1E5) Mouse mAb (Clone previously known as Hepatocyte Specific Antigen) recognizes endogenous levels of total CPS1/Hep Par-1 protein, which is present in the liver and small intestine.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with human liver fixed in formalin.

    Background

    Carbamoyl phosphate synthetase 1 (CPS1), a rate-limiting enzyme in the urea cycle, catalyzes the conversion of ammonia and bicarbonate to carbamoyl phosphate in mitochondria. Non-small cell lung carcinoma (NSCLC) cells with oncogenic KRAS and loss of the tumor suppressor LKB1 express CPS1 and depend on this enzyme for growth. CPS1 maintains pyrimidine/purine balance in these cancer cells. Silencing CPS1 reduces the pyrimidine to purine ratio and stalls DNA synthesis, leading to DNA damage and cancer cell death (1). In addition, the tumor suppressor p53 represses the expression of urea cycle enzymes CPS1, OTC, and ARG1 and, therefore, causes the accumulation of ammonia, suppressing cancer growth (2). Furthermore, research studies suggest that hypermethylation-mediated downregulation of CPS1 expression may contribute to the progression of normal hepatocytes to hepatocellular carcinoma (HCC) (3). CPS1 was identified to be the antigen detected by Hep Par-1 antibody (4).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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