Render Target: STATIC
Render Timestamp: 2024-12-26T11:37:33.630Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-09-30 01:59:16.757
Product last modified at: 2024-12-22T15:45:07.771Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

CREST (E3P4F) Rabbit mAb #81662

Filter:
  • WB
  • IP
  • IF
  • ChIP

    Supporting Data

    REACTIVITY H Mk
    SENSITIVITY Endogenous
    MW (kDa) 60
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • IF-Immunofluorescence 
    • ChIP-Chromatin Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    For optimal ChIP results, use 10 μL of antibody and 10 μg of chromatin (approximately 4 × 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50
    Immunofluorescence (Immunocytochemistry) 1:1600 - 1:6400
    Chromatin IP 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    CREST (E3P4F) Rabbit mAb recognizes endogenous levels of total CREST protein. This antibody does not cross-react with SS18 protein.

    Species Reactivity:

    Human, Monkey

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly168 of human CREST protein.

    Background

    Calcium-responsive transactivator (CREST), also known as SS18L1, is a calcium-dependent neuronal BAF family member expressed in the developing brain (1-4). CREST recruits CBP to nuclear bodies in order to regulate cortical and hippocampal dendrite development (1,5). The activity of CREST is aided by the ATPase of the BAF complex, Brg1, which upon calcium influx, will release a phospho-Rb/HDAC1 repressive complex from neuronal genes (4). CREST mutations have been described in ALS. The protein contains a prion-like domain, associates with the ALS protein FUS, forms aggregates, and is included in paraspeckles (6-8). CREST is highly homologous to SS18 and can similarly form fusions with SSX proteins in synovial sarcomas (9,10).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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