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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

CSDE1 Antibody #62328

Filter:
  • WB
Western Blotting Image 1: CSDE1 Antibody
Western blot analysis of extracts from various cell lines using CSDE1 Antibody (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).

To Purchase # 62328

Cat. # Size Qty. Price Ships
62328S 100 µl
$306 Jan 23

Supporting Data

REACTIVITY H M R Mk
SENSITIVITY Endogenous
MW (kDa) 89
SOURCE Rabbit
Application Key:
  • WB-Western Blotting 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • R-Rat 
  • Mk-Monkey 

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

CSDE1 Antibody recognizes endogenous levels of total CSDE1 protein.

Species Reactivity:

Human, Mouse, Rat, Monkey

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human CSDE1 protein. Antibodies are purified by peptide affinity chromatography.

Background

Originally described as the product of an active transcription unit located upstream of the N-RAS gene (1), CSDE1 (cold shock domain-containing protein E1) is a highly conserved RNA-binding protein containing five cold-shock domains (2,3). CSDE1 is mostly localized in the cytoplasm where it interacts with distinct protein complexes involved in the post-transcriptional regulation of numerous mRNAs in a dynamic and complex manner (4). CSDE1 plays a role in maintaining the undifferentiated state of human embryonic stem cells (hESCs), and its loss results in accelerated neural differentiation and neurogenesis, partially via post-transcriptional regulation of fatty acid binding protein 7 (FABP7) and vimentin (VIM) mRNAs (5). In human melanoma, increased CSDE1 protein expression has been shown to modulate the levels of pro-oncogenic factors such as vimentin or RAC1 and tumor suppressors such as PTEN, thus promoting tumor invasion and metastasis (6).
For Research Use Only. Not For Use In Diagnostic Procedures.
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