Render Target: STATIC
Render Timestamp: 2024-11-20T11:57:52.883Z
Commit: 5c4accf06eb7154018ba3f54329c7590f97f534a
XML generation date: 2024-09-30 01:56:48.707
Product last modified at: 2024-11-19T20:00:09.874Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

CTGF (D8Z8U) Rabbit mAb #86641

Filter:
  • WB
  • IF

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 35
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunofluorescence (Immunocytochemistry) 1:50 - 1:200

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    For a carrier free (BSA and azide free) version of this product see product #92687.

    Protocol

    Specificity / Sensitivity

    CTGF (D8Z8U) Rabbit mAb recognizes endogenous levels of total CTGF protein. By Western blot analysis, the antibody detects a 120 kDa protein of unknown identity in both CTGF-positive and CTGF-negative cell lines.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val174 of human CTGF protein.

    Background

    Connective tissue growth factor (CTGF, CCN2) belongs to the CCN (CYR61, CTGF, NOV) family of secreted extracellular matrix (ECM) proteins (1). Members of this family contain four conserved cysteine-rich domains, and interact in the ECM with a diverse array of cell surface receptors, including integrins and heparin-sulfate proteoglycans (2). These interactions regulate a multitude of cellular and tissue functions, including adhesion, proliferation, migration, differentiation, senescence, angiogenesis, inflammation, and wound repair (1,3-5). The CTGF gene is a transcriptional target of both YAP/TAZ and TGFβ-SMAD signaling pathways (6,7), and aberrant regulation of CTGF expression is strongly associated with pathological conditions, notably cancer and fibrosis (8,9).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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