Render Target: STATIC
Render Timestamp: 2024-12-20T10:59:40.168Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-09-30 01:53:07.351
Product last modified at: 2024-12-17T18:46:37.249Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

CTGF (E2W5M) Rabbit mAb #10095

Filter:
  • WB

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 16-20, 35
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    CTGF (E2W5M) Rabbit mAb recognizes endogenous levels of total CTGF protein. This antibody recognizes both full-length CTGF, and cleaved C-terminal fragments of CTGF, which vary in length (16-20 kDa) depending upon cleavage context.

    Species Reactivity:

    Human

    The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.

    Species predicted to react based on 100% sequence homology:

    Chicken, Bovine

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro265 of human CTGF protein.

    Background

    Connective tissue growth factor (CTGF, CCN2) belongs to the CCN (CYR61, CTGF, NOV) family of secreted extracellular matrix (ECM) proteins (1). Members of this family contain four conserved cysteine-rich domains, and interact in the ECM with a diverse array of cell surface receptors, including integrins and heparin-sulfate proteoglycans (2). These interactions regulate a multitude of cellular and tissue functions, including adhesion, proliferation, migration, differentiation, senescence, angiogenesis, inflammation, and wound repair (1,3-5). The CTGF gene is a transcriptional target of both YAP/TAZ and TGFβ-SMAD signaling pathways (6,7), and aberrant regulation of CTGF expression is strongly associated with pathological conditions, notably cancer and fibrosis (8,9).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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