Render Target: STATIC
Render Timestamp: 2025-02-26T10:50:44.700Z
Commit: a96f9d7628de39acbdefdc43be1285df87057945
XML generation date: 2024-08-01 15:28:51.420
Product last modified at: 2025-01-01T09:04:57.193Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

CUL4A Antibody #2699

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H Mk
    SENSITIVITY Endogenous
    MW (kDa) 80, 82
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    CUL4A Antibody detects endogenous levels of total human CUL4A protein.

    Species Reactivity:

    Human, Monkey

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding residues surrounding Ser12 of human CUL4A. Antibodies are purified by peptide affinity chromatography.

    Background

    Cullin-4 (CUL4) is a member of the cullin family of related ubiquitin ligases (1). A pair of mammalian CUL4 proteins have been identified: CUL4A and CUL4B. The carboxy-terminal CUL4 domain interacts with Rbx1 and E2 enzymes, while the amino-terminal CUL4 domain interacts with the BPB domain of UV-damaged DNA-binding protein DDB1 to form a CUL4-DDB1 ubiquitin ligase complex (2). This CUL4-DDB1 complex binds a variety of WD40-containing proteins that help to enhance additional substrate recruitment (2,3). The CUL4 complex has been shown to target substrates involved in DNA repair, cell cycle progression (2), transcription (3), and development (2-6).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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