CXCL9/MIG (E6Z5W) Rabbit mAb (BSA and Azide Free) #37438
- WB
- IHC
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 15 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IHC-Immunohistochemistry
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier-free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.
Formulation
Supplied in 1X PBS, BSA and Azide Free.
For standard formulation of this product see product #30327.
Storage
Specificity / Sensitivity
CXCL9/MIG (E6Z5W) Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total CXCL9/MIG protein. This antibody does not cross-react with CXCL10, CXCL11, or CXCL2 protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to full-length human CXCL9/MIG protein.
Background
C-X-C motif chemokine ligand 9 (CXCL9, MIG) is a soluble chemokine expressed by multiple cell types, such as endothelial cells, monocytes, and tumor cells. The expression of CXCL9 is upregulated in response to proinflammatory cytokines, such as IFN-γ and TNF-α. (1,2). CXCL9 binds to CXCR3, a GPCR that is expressed on the surface of multiple populations of immune cells, particularly exclusively activated T lymphocytes (3). Binding of CXCL9 to CXCR3 promotes T cell chemotaxis and infiltration into sites of inflammation that form in settings such as viral infection and tumorigenesis (4-6). Research studies have demonstrated production of CXCL9 by tumor cells mediates tumor suppression through the recruitment of tumor antigen-specific T cells (7).
- Shin, S.Y. et al. (2010) J Biol Chem 285, 30731-40.
- Hiroi, M. and Ohmori, Y. (2003) Biochem J 376, 393-402.
- Loetscher, M. et al. (1996) J Exp Med 184, 963-9.
- Tensen, C.P. et al. (1999) J Invest Dermatol 112, 716-22.
- Han, K. et al. (2020) Am J Respir Cell Mol Biol, doi: 10.1165/rcmb.2020-0354OC.
- Russo, E. et al. (2020) J Leukoc Biol 108, 673-685.
- Gorbachev, A.V. et al. (2007) J Immunol 178, 2278-86.
Limited Uses
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