Render Target: STATIC
Render Timestamp: 2024-12-26T10:53:54.768Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-09-30 01:57:50.695
Product last modified at: 2024-12-17T18:59:19.612Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
  1. Products /
  2. Primary Antibodies /
  3. Cyclin C (E6V4Z) Rabbit mAb
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Cyclin C (E6V4Z) Rabbit mAb #68179

Filter:
  • WB
  • IP
  • IF
  • F

    Supporting Data

    REACTIVITY H M
    SENSITIVITY Endogenous
    MW (kDa) 30
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • IF-Immunofluorescence 
    • F-Flow Cytometry 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:100
    Immunofluorescence (Immunocytochemistry) 1:1600 - 1:6400
    Flow Cytometry (Fixed/Permeabilized) 1:100 - 1:400

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Cyclin C (E6V4Z) Rabbit mAb recognizes endogenous levels of total cyclin C protein.

    Species Reactivity:

    Human, Mouse

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro263 of human cyclin C protein.

    Background

    Cyclin dependent kinases (CDKs) are regulated in part through binding to regulatory cyclins. While many cyclin/CDK complexes regulate progression through the cell cycle, others, such as cyclin C/CDK8, regulate transcription and other cellular functions. Cyclin C/CDK8 forms a regulatory sub-complex with Med12 and Med13, which associates with the multi-subunit transcriptional coactivator Mediator Complex to modulate RNA polymerase II-dependent transcription. In addition to its role in transcription, cyclin C is important in the regulation of mitochondrial dynamics, apoptosis, and cell cycle progression (1). Cytosolic cyclin C induces mitochondrial fragmentation and apoptosis, and is required for activation of the Bcl2 family member, Bax (2). Cytoplasmic cyclin C also supports mitochondrial fission through dynamin-related protein 1 (Drp1) in response to oxidative stress (3). Cyclin C acts synergistically with PTEN to maintain levels of tumor suppressors p53 and p21 in a mouse model of thyroid cancer (4). In complex with CDK3, cyclin C regulates Rb-dependent exit from quiescence in human cells (5).

    Database Links

    UniProt ID: P24863


    Entrez-Gene Id: 892


    Limited Uses

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    For Research Use Only. Not For Use In Diagnostic Procedures.
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