Cyclin F (D9K2U) Rabbit mAb #81045
- WB
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 90 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
Cyclin F contains a cyclin box domain in addition to an F-box domain, but does not regulate the activity of cyclin dependent kinases. Cyclin F expression does oscillate during the cell cycle, however, peaking in G2 phase (2).
Cyclin F interacts with the centrosomal protein CP110, which plays critical roles centriole duplication and spindle formation. Cyclin F-mediated degradation of CP110 in G2 phase is required for normal progression into mitosis (3). In response to ionizing radiation, which causes DNA double strand breaks, Cyclin F interacts with B-Myb, preventing cyclin A-dependent phosphorylation of B-Myb, and delaying progression into mitosis. This G2 phase arrest allows the cell to respond to the DNA damage-induced G2/M phase checkpoint (4). Cyclin F also controls the stability of the ribonucleotide reductase M2 subunit, RRM2, which functions in maintaining the levels of dNTPs available in the cell for DNA synthesis and repair, in response to genotoxic stress (5). Researchers have implicated cyclin F as a prognostic marker in hepatocellular carcinoma (HCC) (6).
Limited Uses
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