Render Target: STATIC
Render Timestamp: 2024-11-29T11:07:26.752Z
Commit: cd2fae6ca3f811b1ddb1df24ac291ed56d5d501b
XML generation date: 2024-04-05 20:40:50.164
Product last modified at: 2024-05-30T07:03:58.894Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

Cyclin T1 Antibody #8744

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Inquiry Info. # 8744

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    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 81
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Cyclin T1 Antibody recognizes endogenous levels of total Cyclin T1 protein. This antibody also cross-reacts with a 50 kDa protein of unknown origin in some cell lines.

    Species Reactivity:

    Human

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly348 of human Cyclin T1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    Positive transcription elongation factor (P-TEFb) is a heterodimer composed of cyclin T proteins and CDK9. P-TEFb plays a critical role in the transition of the RNA polymerase II (RNAPII) machinery from transcription initiation to elongation (1). At some genes during transcription initiation, RNAPII moves approximately 50 nucleotides away from the transcription start site into the gene where it then pauses and awaits signaling for the formation of a productive transcription elongation complex (1,2). The release of this promoter proximal pausing of RNAPII is signaled by phosphorylation of the C-terminal domain (CTD) within the largest subunit of RNAPII at Ser2 of the heptapeptide repeat sequence by P-TEFb (3). This phosphorylation event is important for the recruitment of mRNA processing factors and chromatin modifiers that are necessary for proper gene expression (4,5). P-TEFb also promotes transcription elongation by phosphorylating DSIF (DRB-induced stimulating factor) and NELF (negative elongation factor), two negative elongation factors that retain RNAPII at the promoter proximal region of genes to initiate transcription elongation (6,7).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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