Cystathionine γ-Lyase (D4E9J) Rabbit mAb #30068

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Supporting Data

REACTIVITY	H Mk
SENSITIVITY	Endogenous
MW (kDa)	44
Source/Isotype	Rabbit IgG

Application Key:

WB-Western Blotting

Species Cross-Reactivity Key:

H-Human
Mk-Monkey

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Cystathionine γ-Lyase (D4E9J) Rabbit mAb recognizes endogenous levels of total cystathionine γ-lyase protein.

Species Reactivity:

Human, Monkey

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human cystathionine γ-lyase protein.

Background

Cystathionine γ-lyase (CGL) is an enzyme in the transsulfuration pathway, a route in the metabolism of sulfur-containing amino acids (1). This enzyme regulates local vasodilation and blood pressure by generating hydrogen sulfide (H₂S) as a physiological signaling molecule (2). A rodent model of sleep apnea showed that H₂S production by cystathionine γ-lyase in the carotid body triggers hypertension in rodents during intermittent hypoxia, suggesting that inhibition of this enzyme may prevent the hypertension associated with sleep apnea (3). In addition, dietary restriction of sulfur-containing amino acids upregulates hepatic cystathionine γ-lyase expression in mice, leading to elevated production of H₂S and protection from hepatic ischemia perfusion injury, indicating that this enzyme is critical for the benefits of dietary restriction (4).

Database Links

UniProt ID: P32929

Entrez-Gene Id: 1491

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For Research Use Only. Not For Use In Diagnostic Procedures.

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