Render Target: STATIC
Render Timestamp: 2024-11-21T13:13:12.239Z
Commit: 5c4accf06eb7154018ba3f54329c7590f97f534a
XML generation date: 2024-08-01 15:29:17.119
Product last modified at: 2024-05-30T07:09:18.622Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

Cytochrome b Antibody #69202

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H Mk
    SENSITIVITY Endogenous
    MW (kDa) 26
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Cytochrome b Antibody recognizes endogenous levels of total cytochrome b protein.

    Species Reactivity:

    Human, Monkey

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro208 of human cytochrome b protein. Antibodies are purified by peptide affinity chromatography.

    Background

    Cytochrome b, encoded by the gene MT-CYB, is a subunit of ubiquinol-cytochrome c reductase, also known as cytochrome bc1, or complex III, of the mitochondrial respiratory (electron transport) chain (1). This protein complex mediates electron transfer from ubiquinol to cytochrome c, contributing to the generation of a proton gradient that powers ATP synthesis (2). Cytochrome b is an integral membrane protein and notable for being the only protein within complex III that is encoded by the mitochondrial genome. Genetic sequence analyses have identified mutations in MT-CYB that are linked to a variety of diseases and pathologies (3,4). Natural variations in the sequence of MT-CYB have also proven highly useful as a tool to determine phylogenetic relationships between organisms (5).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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