Render Target: STATIC
Render Timestamp: 2024-11-22T11:07:05.520Z
Commit: 5c4accf06eb7154018ba3f54329c7590f97f534a
XML generation date: 2024-09-30 01:53:35.461
Product last modified at: 2024-11-18T14:30:15.547Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77

Cytochrome c (6H2.B4) Mouse mAb #12963

Filter:
  • IP
  • IF
  • F

    Supporting Data

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 14
    Source/Isotype Mouse IgG1
    Application Key:
    • IP-Immunoprecipitation 
    • IF-Immunofluorescence 
    • F-Flow Cytometry 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Immunoprecipitation 1:100
    Immunofluorescence (Immunocytochemistry) 1:300
    Flow Cytometry (Fixed/Permeabilized) 1:200 - 1:400

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Cytochrome c (6H2.B4) Mouse mAb recognizes endogenous levels of total cytochrome c protein by immunofluorescence. This antibody is not recommended for western blot.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with recombinant rat cytochrome c protein.

    Background

    Cytochrome c is a well conserved electron-transport protein and is part of the respiratory chain localized to mitochondrial intermembrane space (1). Upon apoptotic stimulation, cytochrome c released from mitochondria associates with procaspase-9 (47 kDa)/Apaf 1. This complex processes caspase-9 from inactive proenzyme to its active form (2). This event further triggers caspase-3 activation and eventually leads to apoptosis (3).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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