Render Target: STATIC
Render Timestamp: 2024-11-21T13:51:36.718Z
Commit: 5c4accf06eb7154018ba3f54329c7590f97f534a
XML generation date: 2024-05-10 22:34:33.075
Product last modified at: 2024-11-15T13:00:17.206Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

Cytochrome c Antibody #4272

Filter:
  • WB
  • IHC

    Supporting Data

    REACTIVITY H M R Mk Dm
    SENSITIVITY Endogenous
    MW (kDa) 14
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    • IHC-Immunohistochemistry 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 
    • Dm-D. melanogaster 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunohistochemistry (Paraffin) 1:200

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Cytochrome c Antibody detects endogenous levels of cytochrome c protein.

    Species Reactivity:

    Human, Mouse, Rat, Monkey, D. melanogaster

    The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.

    Species predicted to react based on 100% sequence homology:

    Bovine

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro72 of human cytochrome c. Antibodies are purified by protein A and peptide affinity chromatography.     

    Background

    Cytochrome c is a well conserved electron-transport protein and is part of the respiratory chain localized to mitochondrial intermembrane space (1). Upon apoptotic stimulation, cytochrome c released from mitochondria associates with procaspase-9 (47 kDa)/Apaf 1. This complex processes caspase-9 from inactive proenzyme to its active form (2). This event further triggers caspase-3 activation and eventually leads to apoptosis (3).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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