Render Target: STATIC
Render Timestamp: 2025-01-22T05:16:10.787Z
Commit: da7e4f2f0d1aed1f1f8e20e4e2ecab8f33cbd595
XML generation date: 2025-01-15 18:01:10.779
Product last modified at: 2025-01-01T09:00:46.447Z
Cell Signaling Technology Logo

Basket Updated

0

Items added

1% for the planet logo
PDP - Template Name: Antibody Sampler Kit
PDP - Template ID: *******4a3ef3a

Cytokeratin Antibody Sampler Kit #9384

Cytokeratin Antibody Sampler Kit: Image 1
Western blot analysis of extracts from HeLa and MCF7 cells using Keratin 7 (D1E4) XP® Rabbit mAb. Note that MCF7 cells are negative for Keratin 7, as expected.

To Purchase # 9384

Cat. # Size Qty. Price Ships
9384T 1 Kit
6 x 20 microliters
$558
  • Product Includes
  • Related Products
Product IncludesQuantityApplicationsReactivityMW(kDa)Isotype
Keratin 17 (D73C7) Rabbit mAb #454320 µlWB, IF, FH M R48Rabbit IgG
Keratin 8/18 (C51) Mouse mAb #454620 µlWB, IHC, IF, FH Mk46 Keratin 18. 55 Keratin 8.Mouse IgG1
Keratin 18 (DC10) Mouse mAb #454820 µlWB, IHC, IF, FH46Mouse IgG1
Keratin 19 (BA17) Mouse mAb #455820 µlWB, IHCH40Mouse IgG1
Pan-Keratin (C11) Mouse mAb #454520 µlWB, IHC, IF, FH M R Mk46-58Mouse IgG1
Keratin 7 (D1E4) XP® Rabbit mAb #446520 µlWB, IF, FH52Rabbit IgG
Anti-mouse IgG, HRP-linked Antibody #7076100 µlWBMHorse 
Anti-rabbit IgG, HRP-linked Antibody #7074100 µlWBRabGoat 

Product Information

Product Description

The Cytokeratin Antibody Sampler Kit provides an economical means to evaluate the presence and status of selected keratin proteins. The kit provides enough primary and secondary antibodies to perform two Western blot experiments per primary antibody.

Specificity / Sensitivity

The pan-keratin (C11) mouse mAb detects endogenous levels of total keratins 4, 5, 6, 8, 10, 13 and 18. The antibody does not cross-react with other keratins. Each of the remaining antibodies included in this kit detect endogenous levels of the specified keratin protein and do not cross-react with other keratin proteins.

Source / Purification

Pan-Keratin Mouse mAb (C11) is produced by immunizing animals with a cytoskeleton preparation from A431 cells. Keratin 8/18 (C51) Mouse mAb is produced by immunizing animals with a cytoskeleton preparation from HeLa cells. Keratin 18 (DC10) Mouse mAb is produced by immunizing animals with human PMC-42 breast carcinoma cells. Keratin 19 (BA17) Mouse mAb is produced by immunizing animals with detergent-insoluble extract of human mammary epithelial organoids. Keratin 7 (D1E4) XP® Rabbit mAb is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human keratin 7 protein and Keratin 17 (D73C7) XP® Rabbit mAb is produced by immunizing animals with a synthetic peptide corresponding to amino acids near the carboxy terminus of human keratin 17

Background

Keratins (cytokeratins) are intermediate filament proteins that are mainly expressed in epithelial cells. Keratin heterodimers composed of an acidic keratin (or type I keratin, keratins K9-K28) and a basic keratin (or type II keratin, keratins K1-K8 and K71-K80) assemble to form filaments. Keratin isoforms demonstrate tissue- and differentiation-specific profiles that make them useful as research and clinical biomarkers (1,2).

Dysregulation/mutations in keratin genes can lead to a variety of disorders affecting the skin, hair, nails, and other epithelial tissues (3). While expression of keratins can be variable, immunohistochemical staining of keratins is widely used to help in the identification and classification of epithelial tumors, and may also provide prognostic information.

Keratins 8 and 18 (K8/K18) are expressed in simple epithelia of normal tissue, as well as in adenocarcinomas of the breast, lung, ovary, and gastrointestinal tract. Keratin 17 is expressed in basal keratinocytes of stratified epithelia, hair follicles, and sebaceous glands. Onset of keratin 17 expression coincides with the definition of major epithelial lineages during skin development (4). Keratin 14 (K14) is expressed in basal cells of stratified epithelia, and in basal-like subtypes of breast cancer and squamous cell carcinomas. Keratin 19 (K19) is expressed in glandular epithelia, including the liver, gallbladder, and pancreas, as well as in adenocarcinomas of the breast, thyroid, and bile duct. Keratin 20 (K20) is expressed in gastrointestinal epithelium, urothelium, and Merkel cells in the skin, as well as in colorectal carcinomas and some urothelial carcinomas. Keratin 5/6 (K5/6) is expressed in basal cells of stratified epithelia, including the skin, prostate, and breast, as well as in basal-like breast cancers, squamous cell carcinomas, and some lung carcinomas. Keratin 7 (K7) is expressed in glandular epithelia, such as those in the lung, breast, and female reproductive tract, as well as in adenocarcinomas of the lung, breast, and ovary (5,6).

Keratins, particularly K8, K18, and K19, serve as biomarkers for identification of circulating tumor cells (CTCs) (5).

Post-translational modifications, including phosphorylation, acetylation, ubiquitylation, sumoylation, glycosylation, and transamidation, have been shown to affect the functions of keratins in normal and disease states (6). Understanding the molecular mechanisms underlying these PTMs may provide insights into cancer pathogenesis.
For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit our Trademark Information page.