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XML generation date: 2024-05-10 06:24:31.498
Product last modified at: 2025-01-01T09:06:32.097Z
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PDP - Template Name: Antibody Duet
PDP - Template ID: *******ad0fa02

PhosphoPlus® DARPP-32 (Thr34) Antibody Duet #24591

PhosphoPlus® DARPP-32 (Thr34) Antibody Duet: Image 1
Western blot analysis of extracts from MKN-45 cells, untreated (-) or treated with Forskolin #3828 (30 μM, 20 min; +), or nonphospho-DARPP-32 and phospho-DARPP-32 (Thr34) recombinant proteins using Phospho-DARPP-32 (Thr34) (D27A4) Rabbit mAb (upper) or DARPP-32 (19A3) Rabbit mAb #2306 (lower).

To Purchase # 24591

Cat. #

Size

24591S
1 Kit
  • Product Includes
  • Related Products
Product IncludesQuantityReactivityMW(kDa)Isotype
Phospho-DARPP-32 (Thr34) (D27A4) Rabbit mAb #12438100 µlH32Rabbit IgG
DARPP-32 (19A3) Rabbit mAb #2306100 µlM R32Rabbit 

Product Information

Product Description

PhosphoPlus® Duets from Cell Signaling Technology (CST) provide a means to assess protein activation status. Each Duet contains an activation-state and total protein antibody to your target of interest. These antibodies have been selected from CST's product offering based upon superior performance in specified applications.

Background

DARPP-32 (dopamine and cyclic AMP-regulated phosphoprotein, relative molecular mass 32,000) is a cytosolic protein highly enriched in medium-sized spiny neurons of the neostriatum (1). It is a bifunctional signaling molecule that controls serine/threonine kinase and serine/threonine phosphatase activity (2). Dopamine stimulates phosphorylation of DARPP-32 through D1 receptors and activation of PKA. PKA phosphorylation of DARPP-32 at Thr34 converts it into an inhibitor of protein phosphatase 1 (1). DARPP-32 is converted into an inhibitor of PKA when phosphorylated at Thr75 by cyclin-dependent kinase 5 (CDK5) (2). Mice containing a targeted deletion of the DARPP-32 gene exhibit an altered biochemical, electrophysiological, and behavioral phenotype (3).

Pathways

Explore pathways related to this product.


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