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Daxx Antibody #4532

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Filter:
  • WB
  • IP
Western Blotting Image 1: Daxx Antibody
Western blot analysis of extracts from Ramos and THP-1 cells, using Daxx Antibody.
This product is discontinued

Inquiry Info. # 4532

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Supporting Data

REACTIVITY H M R
SENSITIVITY Endogenous
MW (kDa) 120
SOURCE Rabbit
Application Key:
  • WB-Western Blotting 
  • IP-Immunoprecipitation 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • R-Rat 

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:100

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Daxx Antibody detects endogenous levels of total Daxx protein.

Species Reactivity:

Human, Mouse, Rat

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to a region around residue 260 of Daxx. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Daxx is a ubiquitously expressed protein that was originally identified through a yeast two-hybrid screen as an interactor with the cytoplasmic domain of Fas. It was found to enhance Fas-mediated apoptosis and activate the JNK pathway (1). However, additional studies have revealed that Daxx is actually a nuclear protein localizing to promyelocytic leukemia oncogenic domains (PODs) (2,3). Nuclear interactions have since been observed with CENP-C (4), Pax3 (5), DNA methyltransferase I (6) and chromatin-associated proteins, including histone deacetylase II, H2A, H2B, H3, H4 and Dek (5). Roles for Daxx have been suggested in transcriptional repression and cell cycle control. Loss of Daxx in mice leads to embryonic lethality with extensive developmental apoptosis, suggesting a role for Daxx directly or indirectly in suppressing cell death (5). Furthermore, inhibition of Daxx expression using RNAi has confirmed Daxx to be anti-apoptotic and to repress transcriptional activity of targets including NF-κB and E2F-1 (7).

Pathways

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For Research Use Only. Not For Use In Diagnostic Procedures.
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