Render Target: STATIC
Render Timestamp: 2024-11-22T11:19:19.073Z
Commit: 5c4accf06eb7154018ba3f54329c7590f97f534a
XML generation date: 2024-11-12 23:02:08.614
Product last modified at: 2024-11-20T09:00:12.961Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

DCAF16 (F8E1P) Rabbit mAb #52485

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 25
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    DCAF16 (F8E1P) Rabbit mAb recognizes endogenous levels of total DCAF16 protein. This antibody may cross-react with proteins of unknown origin around 50 kDa and 100 kDa.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human DCAF16 protein.

    Background

    The CUL4-DDB1 ubiquitin ligase complex mediates the degradation of target proteins involved in various cellular processes, including DNA damage repair, cell cycle progression, transcription, and development (1-5). DDB1- and CUL4-associated factor 16 (DCAF16) is an E3 ubiquitin ligase that, like other members of the DCAF family, functions as a substrate receptor to recruit the CUL4-DDB1 complex to target proteins for ubiquitination (1,6). DCAF16 is often studied in the context of targeted protein degradation (TPD), where it is utilized to specifically mark disease-associated nuclear proteins for ubiquitination and removal (7). For instance, DCAF16 was found to mediate degradation of FKBP12 and BRD4 protein following treatment with heterobifunctional degrader compounds (6).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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