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Product last modified at: 2025-01-01T09:05:49.080Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

DCC (E4U1X) Rabbit mAb #17460

Filter:
  • WB
Western Blotting Image 1: DCC (E4U1X) Rabbit mAb
Western blot analysis of extracts from A-673, MOLP-8, and 22Rv1 cells using DCC (E4U1X) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). Negative expression of DCC protein in 22Rv1 cells is consistent with the predicted expression pattern.

To Purchase # 17460

Cat. # Size Qty. Price Ships
17460T 20 µl
$156
17460S 100 µl
$371

Supporting Data

REACTIVITY H
SENSITIVITY Endogenous
MW (kDa) 185
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
Species Cross-Reactivity Key:
  • H-Human 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Simple Western™ 1:10 - 1:50

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

DCC (E4U1X) Rabbit mAb recognizes endogenous levels of total DCC protein.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser1200 of human DCC protein.

Background

Deleted in colorectal cancer (DCC) is a type I transmembrane protein belonging to the immunoglobulin superfamily. Originally implicated as a tumor suppressor gene given its lack of expression in colorectal carcinomas, DCC was later characterized as a receptor for netrin (1). Netrin proteins belong to an evolutionarily conserved family of laminin-like molecules that play an essential role in axon guidance during development. These secreted proteins can have opposing functions depending on specific receptor associations. Binding of netrin-1 by DCC on commissural axons mediates growth cone attraction, while binding by uncoordinated 5 (UNC5) family receptors induces repulsion (2,3). In addition to axon guidance, DCC is also suggested to regulate tissue organization, cell adhesion, motility, proliferation, differentiation, and cell survival via its binding activity with netrin-1 (1,3). Recent findings suggest that reduced expression of netrin-1 or DCC can lead to dopaminergic (DA) neuron death within the substantia nigra pars compacta (SNpc) of the midbrain, an area that is particularly vulnerable to degeneration in Parkinson's disease (PD). Conversely, overexpression of netrin-1 in animal models of PD has been shown to be neuroprotective, suggesting that the netrin-1/DCC ligand/receptor pair may be a therapeutic target for this neurodegenerative disease (4,5).
For Research Use Only. Not For Use In Diagnostic Procedures.
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