Render Target: STATIC
Render Timestamp: 2025-03-18T11:44:40.759Z
Commit: 779953b12a5930618aae6aca7c87fb286faeb1d7
XML generation date: 2025-03-07 13:06:53.526
Product last modified at: 2025-03-06T14:45:15.920Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

DCP1B (D2P9W) Rabbit mAb #13233

Filter:
  • WB
  • IP
  • IF

    Supporting Data

    REACTIVITY H Mk
    SENSITIVITY Endogenous
    MW (kDa) 75
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • H-Human 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50
    Immunofluorescence (Immunocytochemistry) 1:100

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    DCP1B (D2P9W) Rabbit mAb recognizes endogenous levels of total DCP1B protein. This antibody may cross-react with DCP1A.

    Species Reactivity:

    Human, Monkey

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val202 of human DCP1B protein.

    Background

    mRNA decapping is an important process in the mRNA turnover (1). DCP1A and DCP2 were identified as two human decapping enzymes and homologs of the better-characterized S. cerevisiae enzymes. Both putative decapping enzymes interact with the regulator of nonsense transcripts 1 (UPF1) and may be recruited by UPF1 or related proteins to mRNA sequences that contain premature termination codons (1). Additional research studies demonstrate that DCP1A, DCP1B (the homolog of DCP1A) and DCP2 colocalize with decapping activation factors RCK/p54 and Lsm proteins in cytoplasmic loci (2). DCP1A, DCP1B and DCP2 are components of cytoplasmic processing (P) bodies, with hyper-phosphorylation of DCP1A during mitosis suggesting a possible mechanism of P-body regulation during the cell cycle (3,4).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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