DCPS (E3A4Q) Rabbit mAb #70542
- WB
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 39 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Protocol
Specificity / Sensitivity
DCPS (E3A4Q) Rabbit mAb recognizes endogenous levels of total DCPS protein.
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu140 of human DCPS protein.
Background
DCPS is a scavenger pyrophosphate and a member of the histidine triad family, which breaks down the mRNA cap structure during the decay process. During 3’-5’ mRNA decay, DCPS hydrolyzes m(7)GTP to generate m7GMP (1,2). DCPS also binds m(7)GDP strongly, which inhibits its activity against m(7)GTP (3). DCPS can shuttle from the nucleus to the cytoplasm and also plays a role in pre-mRNA splicing by affecting the nuclear cap-binding protein CBP20 (4). Additionally, DCPS can contribute to miRNA turnover, a function that is independent of decaying activity and dependent on exonuclease XRN2 (5). mRNA cap analogs that are resistant to DCPS activity have been widely utilized in the study of mRNA decay and stability (6-8). Inhibitors of decapping enzymes have been shown to be effective in spinal muscular atrophy mouse models (9).
- Liu, H. et al. (2002) EMBO J 21, 4699-708.
- van Dijk, E. et al. (2003) Proc Natl Acad Sci USA 100, 12081-6.
- Wypijewska, A. et al. (2012) Biochemistry 51, 8003-13.
- Shen, V. et al. (2008) RNA 14, 1132-42.
- Meziane, O. et al. (2015) Sci Rep 5, 16688.
- Grudzien, E. et al. (2006) J Biol Chem 281, 1857-67.
- Kowalska, J. et al. (2008) RNA 14, 1119-31.
- Rydzik, A.M. et al. (2017) Nucleic Acids Res 45, 8661-8675.
- Gogliotti, R.G. et al. (2013) Hum Mol Genet 22, 4084-101.
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