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Render Timestamp: 2024-09-26T11:01:18.730Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

DDR2 Antibody #12133

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Inquiry Info. # 12133

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    Supporting Data

    REACTIVITY H R
    SENSITIVITY Endogenous
    MW (kDa) 120-140
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:100

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    DDR2 Antibody detects endogenous levels of total DDR2 protein. This antibody also cross-reacts with 60 and 80 kDa proteins of unknown origin.

    Species Reactivity:

    Human, Rat

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu491 of human DDR2 protein. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    The discoidin domain receptors (DDRs) are receptor tyrosine kinases with a discoidin homology repeat in their extracellular domains, activated by binding to extracellular matrix collagens. So far, two mammalian DDRs have been identified: DDR1 and DDR2 (1). They are widely expressed in human tissues and may have roles in smooth muscle cell-mediated collagen remodeling (2). Research studies have implicated aberrant expression and signaling of DDRs in human diseases related to increased matrix degradation and remodeling, such as cardiovascular disease, liver fibrosis, and tumor invasion (1).
    Injection of colon tumor cells into DDR2 knock out mice predisposes the hepatic stellate cells to colon tumor metastasis (3).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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