Render Target: STATIC
Render Timestamp: 2024-12-26T11:52:51.480Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-12-03 23:01:07.801
Product last modified at: 2024-12-10T09:00:11.614Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

DDX15 (F4H6R) Rabbit mAb #97849

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 91
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:100

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    DDX15 (F4H6R) Rabbit mAb recognizes endogenous levels of total DDX15 protein. This antibody detects a 45 kDa band of unknown origin in some cell lines.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human DDX15 protein.

    Background

    DEAH-box helicase 15 (DHX15), also known as DEAD-box polypeptide 15 (DDX15), is an RNA helicase involved in mRNA processing and antiviral innate immunity (1-4). It is a ubiquitously expressed protein containing a conserved DEAD (Asp-Glu-Ala-Asp) motif, which is important for ATP hydrolysis and unwinding RNA structures (1-4). DDX15 acts as an important RNA sensor in myeloid dendritic cells and induces the production of type I IFN and proinflammatory cytokines (4,5). In intestinal epithelial cells, DDX15 interacts with NLRP6 to restrict viral infection by triggering NLRP6 inflammasome activation and IL-18 secretion (5).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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