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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

DDX21 Antibody #75762

Filter:
  • WB
  • IP
Western Blotting Image 1: DDX21 Antibody
Western blot analysis of extracts from various cell lines using DDX21 Antibody. As expected, mouse DDX21 is detected at a higher molecular weight.

To Purchase # 75762

Cat. # Size Qty. Price
75762S 100 µl
$306

Supporting Data

REACTIVITY H M R Mk
SENSITIVITY Endogenous
MW (kDa) 100
SOURCE Rabbit
Application Key:
  • WB-Western Blotting 
  • IP-Immunoprecipitation 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • R-Rat 
  • Mk-Monkey 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:50

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

DDX21 Antibody recognizes endogenous levels of total DDX21 protein.

Species Reactivity:

Human, Mouse, Rat, Monkey

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala780 of human DDX21 protein. Antibodies are purified by peptide affinity chromatography.

Background

DDX21, also known as RNA helicase II/Gu, is an RNA helicase that plays important roles in transcription and ribosomal RNA (rRNA) processing (1-3). DDX21 associates with many RNA Polymerase I and II genes, including the direct binding to the rDNA locus to promote ribosome biogenesis. The helicase activity of DDX21 contributes to transcription by helping release P-TEFb from the 7SK small nuclear ribonucleoprotein (snRNP) complex (3). Nucleolar localization of DDX21 has been shown to be regulated through phosphorylation by JNK and interaction with c-Jun. Depletion of either c-Jun or JNK has shown to impair rRNA processing (4,5). DDX21 can also become acetylated by CBP, which reduces its activity. Deacetylation by SIRT7 increases DDX21 helicase activity to prevent R-loop accumulation and protect genomic stability (6). DDX21 can also form a complex with DDX1, DHX36, and TRIF to sense dsRNA, which in turn activates interferon response in innate immunity (7,8).
For Research Use Only. Not For Use In Diagnostic Procedures.
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