Decorin (E2N2C) XP® Rabbit mAb #85786
- WB
- IHC
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 40, 100 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IHC-Immunohistochemistry
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunohistochemistry (Paraffin) | 1:100 - 1:400 |
Storage
For a carrier free (BSA and azide free) version of this product see product #55868.
Protocol
Specificity / Sensitivity
Decorin (E2N2C) XP® Rabbit mAb recognizes endogenous levels of total decorin protein. This protein is heavily glycosylated, therefore, multiple bands or a smear may result from western blot analysis using this antibody.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro216 of human decorin protein.
Background
Decorin, named for its role in “decorating” type I collagen fibrils, is a collagen-binding proteoglycan that belongs to the small leucine-rich proteoglycan (SLRP) family (1). Through its binding to type I collagens and other important matrix proteins (e.g., fibronectin, thrombospondin, TGF-β), decorin plays a critically important role in assembly, maintenance, and physiological functions of the extracellular matrix. Notably, multiple research studies have reported anti-tumor functions for decorin and the related protein biglycan (2-4), implicating SLRP proteins as functionally important components of the tumor microenvironment (5). The anti-tumor effects of decorin have been ascribed to its interactions with cell surface receptors and associated signaling pathways (6), including those mediating inflammation, autophagy, and cell proliferation (7-9).
- Neill, T. et al. (2012) Am J Pathol 181, 380-7.
- Neill, T. et al. (2015) Mol Cell Oncol 2, e975645.
- Horváth, Z. et al. (2014) Matrix Biol 35, 194-205.
- Reszegi, A. et al. (2020) Biomolecules 10, 1199. doi: 10.3390/biom10081199.
- Baghy, K. et al. (2020) Adv Exp Med Biol 1272, 17-38.
- Xie, C. et al. (2022) Am J Physiol Cell Physiol 322, C554-C566.
- Schaefer, L. et al. (2017) FEBS J 284, 10-26.
- Merline, R. et al. (2011) Sci Signal 4, ra75.
- Gubbiotti, M.A. et al. (2015) Matrix Biol 48, 14-25.
Limited Uses
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