Render Target: STATIC
Render Timestamp: 2024-11-26T11:45:53.482Z
Commit: d79925545b26f8827f92d145dadc6f0527debdb1
XML generation date: 2024-08-01 15:32:24.422
Product last modified at: 2024-09-20T13:00:11.101Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

Dectin-1/Clec7a Antibody #23042

Filter:
  • WB

    Supporting Data

    REACTIVITY M
    SENSITIVITY Endogenous
    MW (kDa) 10, 22
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • M-Mouse 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Dectin-1/Clec7a Antibody recognizes endogenous levels of total Dectin-1 protein.

    Species Reactivity:

    Mouse

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His26 of mouse Dectin-1 protein. Antibodies are purified by peptide affinity chromatography.

    Background

    Dectin-1, also known as Clec7a, is a C-type lectin receptor expressed by macrophages, monocytes, dendritic cells, neutrophils, and a subset of γδ T cells (1,2). Dectin-1 is a glycoprotein with eight different isoforms, generated through alternative splicing (3-5). It plays an important role in anti-fungal immunity by acting as a pattern recognition receptor for β-glucans found on the cell wall of fungi and some bacteria (5,6). Dectin-1 is composed of a short amino-terminal cytoplasmic domain containing an ITAM-like motif, a transmembrane domain, and an extracellular carboxy-terminal C-type lectin domain (5). Dectin-1 recognizes β-glucans through its C-type lectin domain and transduces signals through its ITAM-like motif by recruiting and activating Syk (7,8). Dendritic cells activated through Dectin-1 promote differentiation of Th17 cells by producing IL-6 and IL-23 (9).

    Dectin-1 may also play a role in neurodegenerative diseases. CLEC7A, the gene that encodes Dectin-1, is upregulated in microglia, the resident macrophages of the brain, in mouse neurodegenerative disease models, including Alzheimer’s disease (10).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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