Render Target: STATIC
Render Timestamp: 2024-12-26T11:43:20.310Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-09-30 01:59:49.093
Product last modified at: 2024-12-19T14:15:22.305Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Dectin-1/ Clec7a (E3P5W) Rabbit mAb #30260

Filter:
  • WB
  • IHC
  • IF

    Supporting Data

    REACTIVITY M
    SENSITIVITY Endogenous
    MW (kDa) 10, 22
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IHC-Immunohistochemistry 
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • M-Mouse 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunohistochemistry (Paraffin) 1:300 - 1:1200
    Immunofluorescence (Frozen) 1:400 - 1:1600

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    For a carrier free (BSA and azide free) version of this product see product #78117.

    Protocol

    Specificity / Sensitivity

    Dectin-1/Clec7a (E3P5W) Rabbit mAb recognizes endogenous levels of total Dectin-1/Clec7a protein.

    Species Reactivity:

    Mouse

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro29 of mouse Dectin-1/Clec7a protein.

    Background

    Dectin-1, also known as Clec7a, is a C-type lectin receptor expressed by macrophages, monocytes, dendritic cells, neutrophils, and a subset of γδ T cells (1,2). Dectin-1 is a glycoprotein with eight different isoforms, generated through alternative splicing (3-5). It plays an important role in anti-fungal immunity by acting as a pattern recognition receptor for β-glucans found on the cell wall of fungi and some bacteria (5,6). Dectin-1 is composed of a short amino-terminal cytoplasmic domain containing an ITAM-like motif, a transmembrane domain, and an extracellular carboxy-terminal C-type lectin domain (5). Dectin-1 recognizes β-glucans through its C-type lectin domain and transduces signals through its ITAM-like motif by recruiting and activating Syk (7,8). Dendritic cells activated through Dectin-1 promote differentiation of Th17 cells by producing IL-6 and IL-23 (9).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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