DEK (E1L3V) Rabbit mAb #13962
- WB
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 50 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.
Species predicted to react based on 100% sequence homology:
Source / Purification
Background
The DEK proto-oncogene functions as a negative regulator of cellular differentiation, senescence, and apoptosis. DEK is translocated and/or over-expressed in a number of different cancers, including acute myeloid leukemia, breast cancer, cervical cancer, hepatocellular carcinoma, melanoma, and small cell lung cancer (1,2). In addition to the role of DEK in cancer biology, which is mainly related to its intracellular functions, extracellular DEK is implicated in the pathogenesis of autoimmune disorders (1,2). Circulating autoantibodies to DEK have been identified in the serum of patients with autoimmune diseases, including juvenile idiopathic arthritis, sarcoidosis, and systemic lupus erythematosus. DEK is secreted by human monocyte-derived macrophages and apoptotic T-lymphocytes and can act as a chemotactic, pro-inflammatory factor (7,8). Exogenous DEK can penetrate neighboring cells, and translocate to the nucleus to carry out its endogenous nuclear functions (9). IL-8 induced secretion of DEK from macrophages serves as a chemoattractant for peripheral blood leukocytes (7).
- Broxmeyer, H.E. et al. (2013) Stem Cells 31, 1447-53.
- Riveiro-Falkenbach, E. and Soengas, M.S. (2010) Clin Cancer Res 16, 2932-8.
- Kappes, F. et al. (2011) Genes Dev 25, 673-8.
- Sammons, M. et al. (2006) J Biol Chem 281, 26802-12.
- McGarvey, T. et al. (2000) J Cell Biol 150, 309-20.
- Soares, L.M. et al. (2006) Science 312, 1961-5.
- Mor-Vaknin, N. et al. (2006) Mol Cell Biol 26, 9484-96.
- Kappes, F. et al. (2008) Mol Cell Biol 28, 3245-57.
- Saha, A.K. et al. (2013) Proc Natl Acad Sci U S A 110, 6847-52.
Limited Uses
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