Desmoglein 3 (5G11) Mouse mAb #20483
- WB
- IF
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 130 |
| Source/Isotype | Mouse IgG1 kappa |
Application Key:
- WB-Western Blotting
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Simple Western™ | 1:10 - 1:50 |
| Immunofluorescence (Immunocytochemistry) | 1:400 - 1:1600 |
Storage
Protocol
Specificity / Sensitivity
Desmoglein 3 (5G11) Mouse mAb recognizes endogenous levels of total desmoglein 3 protein. This antibody does not cross-react with desmoglein 1 or desmoglein 2 proteins.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the sequence of human desmoglein 3 protein.
Background
Desmogleins are calcium-binding transmembrane glycoprotein components of desmosomes, which are intercellular junctions that mediate strong cell-cell adhesion and are linked to the intermediate filament cytoskeleton. Dysregulation of desmosomal protein expression affects both cell adhesion and communication, and has been shown to result in various human pathologies (1). Desmoglein 3 (DSG3) expression is altered in some human cancers, and its expression may be linked to tumor progression (2). Auto-antibodies against desmoglein 1 and desmoglein 3 cause Pemphigus, a life-threatening autoimmune disorder that results in blistering of the skin and mucous membranes (3).
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