Render Target: STATIC
Render Timestamp: 2024-11-04T11:00:17.259Z
Commit: 23cb9f61fe67e1e9093fd644a533c4ff516a6463
XML generation date: 2024-09-30 01:59:39.927
Product last modified at: 2024-09-30T08:01:41.014Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77

Desmoglein 3 (5G11) Mouse mAb #20483

Filter:
  • WB
  • IF

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 130
    Source/Isotype Mouse IgG1 kappa
    Application Key:
    • WB-Western Blotting 
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Simple Western™ 1:10 - 1:50
    Immunofluorescence (Immunocytochemistry) 1:400 - 1:1600

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Desmoglein 3 (5G11) Mouse mAb recognizes endogenous levels of total desmoglein 3 protein. This antibody does not cross-react with desmoglein 1 or desmoglein 2 proteins.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the sequence of human desmoglein 3 protein.

    Background

    Desmogleins are calcium-binding transmembrane glycoprotein components of desmosomes, which are intercellular junctions that mediate strong cell-cell adhesion and are linked to the intermediate filament cytoskeleton. Dysregulation of desmosomal protein expression affects both cell adhesion and communication, and has been shown to result in various human pathologies (1). Desmoglein 3 (DSG3) expression is altered in some human cancers, and its expression may be linked to tumor progression (2). Auto-antibodies against desmoglein 1 and desmoglein 3 cause Pemphigus, a life-threatening autoimmune disorder that results in blistering of the skin and mucous membranes (3).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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