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DHODH Antibody

DHODH Antibody #80981

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Supporting Data

REACTIVITY	H
SENSITIVITY	Endogenous
MW (kDa)	43
SOURCE	Rabbit

Application Key:

WB-Western Blotting

Species Cross-Reactivity Key:

H-Human

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

DHODH Antibody recognizes endogenous levels of total DHODH protein.

Species Reactivity:

Human

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly301 of human DHODH protein. Antibodies are purified by peptide affinity chromatography.

Background

Dihydroorotate dehydrogenase (DHODH) catalyzes the conversion of dihydroorotate to orotate in pyrimidine biosynthesis. DHODH was shown to be a metabolic regulator of myeloid differentiation in AML models. Inhibition of this enzyme triggers myeloid differentiation, decreases leukemic cell burden, and prolongs survival (1). In addition, research studies show that pyrimidine biosynthesis mediated by DHODH is critical for tumorigenesis. Cancer cells deleted of DHODH fail to form tumors, suggesting DHODH as a potential target in cancer therapy (2). Further studies also indicate that loss of DHODH diminishes tumor growth in vivo (3).

Database Links

UniProt ID: Q02127

Entrez-Gene Id: 1723

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For Research Use Only. Not For Use In Diagnostic Procedures.

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