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Render Timestamp: 2024-07-26T10:24:03.315Z
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PDP - Template Name: Monoclonal Antibody (Epitope Tag)
PDP - Template ID: *******f943b5c
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Digoxigenin (D8Q9J) Rabbit mAb #14682

Filter:
  • WB

    Supporting Data

    REACTIVITY
    SENSITIVITY Transfected Only
    MW (kDa)
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:20000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Digoxigenin (D8Q9J) Rabbit mAb recognizes digoxigenin conjugated molecules.

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with KLH-conjugated digoxigenin.

    Background

    Digoxigenin (DIG) is a highly immunogenic steroid derived from “foxglove” plants of the genus Digitalis (1). This small hapten molecule can be covalently linked to proteins, nucleic acids, compounds, and glycans. Antibodies targeted against the DIG hapten display high binding affinity to DIG-conjugated molecules and can be used in molecular biology assays, including in situ hybridization, western blot, ELISA, and TUNEL assays (2-4).

    For Research Use Only. Not For Use In Diagnostic Procedures.
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