DJ-1 (E9T6J) Mouse mAb #35743
- WB
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 22 |
| Source/Isotype | Mouse IgG3 |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Protocol
Specificity / Sensitivity
DJ-1 (E9T6J) Mouse mAb recognizes endogenous levels of total DJ-1 protein.
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys148 of human DJ-1 protein.
Background
Parkinson's disease (PD) is characterized by the presence of Lewy bodies (intracellular inclusions) and by the loss of dopaminergic neurons. Research studies have shown that mutations in α-synuclein, Parkin, and DJ-1 are linked to PD (1). α-synuclein is a major component of the aggregates found in Lewy bodies. Parkin is involved in protein degradation through the ubiquitin-proteasome pathway, and investigators have shown that mutations in Parkin cause early onset of PD (1). Loss-of-function mutations in DJ-1 cause early onset of PD, but DJ-1 is associated with multiple functions: it cooperates with Ras to increase cell transformation, it positively regulates transcription of the androgen receptor, and it may function as an indicator of oxidative stress (2-5). Dopamine D2 receptor-mediated functions are greatly impaired in DJ-1 (-/-) mice, resulting in reduced long-term depression (6).
- Borrelli, E. (2005) Neuron 45, 479-81.
- Bonifati, V. et al. (2003) Science 299, 256-9.
- Nagakubo, D. et al. (1997) Biochem. Biophys. Res. Commun. 231, 509-13.
- Takahashi, K. et al. (2001) J. Biol. Chem. 276, 37556-63.
- Mitsumoto, A. and Nakagawa, Y. (2001) Free Radic. Res. 35, 885-93.
- Goldberg, M.S. et al. (2005) Neuron 45, 489-96.
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