Render Target: STATIC
Render Timestamp: 2024-12-17T11:49:20.639Z
Commit: ff25cf0788e69a87df3da505ebb7b292b97eec1a
XML generation date: 2024-09-30 01:53:20.686
Product last modified at: 2024-12-06T20:30:08.137Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
  1. Products /
  2. Primary Antibodies /
  3. DLAT (4A4-B6-C10) Mouse mAb

DLAT (4A4-B6-C10) Mouse mAb #12362

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  • WB
  • IP
  • IF

    Supporting Data

    REACTIVITY H Mk
    SENSITIVITY Endogenous
    MW (kDa) 69
    Source/Isotype Mouse IgG1
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • H-Human 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50
    Immunofluorescence (Immunocytochemistry) 1:50 - 1:100

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    DLAT (4A4-B6-C10) Mouse mAb recognizes endogenous levels of total DLAT protein.

    Species Reactivity:

    Human, Monkey

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a recombinant human DLAT protein.

    Background

    Dihydrolipoamide acetyltransferase (DLAT) transfers an acetyl group from pyruvate to CoA to synthesize acetyl-CoA (1-4). This protein, also known as the E2 subunit of the pyruvate dehydrogenase complex (PDC-E2), has been implicated in the literature as the primary autoantigen in primary biliary cirrhosis (2-5). Antimitochondrial antibodies (AMAs) are likely formed when DLAT is exposed to the immune system in apoptotic cells of the bile duct (3,5). Research studies have shown that in some cases, cosmetics, NSAIDs, chewing gum, acetaminophen, and other compounds could trigger exposure of DLAT in sensitive individuals (3). The presence of AMAs is often detectable before disease diagnosis (4,5). Research studies have also shown that activation of the Toll-like receptor-3 (TLR-3) pathway is involved in the progression from a subclinical to clinical state (4).

    Database Links

    UniProt ID: P10515


    Entrez-Gene Id: 1737


    Limited Uses

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    For Research Use Only. Not For Use In Diagnostic Procedures.
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