Render Target: STATIC
Render Timestamp: 2024-11-21T12:49:08.600Z
Commit: 5c4accf06eb7154018ba3f54329c7590f97f534a
XML generation date: 2024-08-01 15:24:39.594
Product last modified at: 2024-11-14T14:45:11.276Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

DLST Antibody #5556

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H M R Mk
    SENSITIVITY Endogenous
    MW (kDa) 50
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    DLST Antibody detects endogenous levels of total DLST protein.

    Species Reactivity:

    Human, Mouse, Rat, Monkey

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro188 of human DLST protein. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    α-ketoglutarate dehydrogenase complex is a rate-regulating enzyme in the Krebs Cycle (1). Dihydrolipoamide succinyltransferase (DLST) is a key subunit in this complex (2). Reduction of DLST increases reactive oxygen species production, suggesting its role in oxidative stress (2). Research has shown that deficiency of DLST in mice is linked to increased oxidative stress in mitochondria, a process that may be involved in the pathogenesis of Alzheimer's disease (2).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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