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Render Timestamp: 2024-11-21T13:21:03.592Z
Commit: 5c4accf06eb7154018ba3f54329c7590f97f534a
XML generation date: 2024-09-20 06:14:51.636
Product last modified at: 2024-09-20T07:11:16.643Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464
  1. Products /
  2. Primary Antibodies /
  3. DMAP1 Antibody

DMAP1 Antibody #13326

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H M R Mk
    SENSITIVITY Endogenous
    MW (kDa) 60
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    DMAP1 Antibody recognizes endogenous levels of total DMAP1 protein.

    Species Reactivity:

    Human, Mouse, Rat, Monkey

    The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.

    Species predicted to react based on 100% sequence homology:

    Hamster, Zebrafish, Bovine, Dog

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly121 of human DMAP1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    DNA methyltransferase 1 (DNMT1)-associated protein 1 (DMAP1) is a nuclear protein that functions in transcriptional repression and DNA repair. DMAP1 was first identified as an activator of DNMT1 methyltransferase activity (1). Both DMAP1 and DNMT1 are targeted to replication foci during S phase and function to transfer proper methylation patterns to newly synthesized DNA during replication (1). In late S phase, DMAP1-DNMT1 co-operate with a p33ING1-Sin3-HDAC2 complex to maintain pericentric heterochromatin by deacetylating histones, methylating histone H3 at Lys9, and methylating DNA (1,2). The DMAP1 protein is also part of the TIP60-p400 complex, a histone acetyltransferases (HAT) and chromatin-remodeling complex that functions in DNA repair (3,4). Upon DNA damage, the TIP60-p400 complex acetylates histone H4 at Lys16 to induce chromatin relaxation and activation of the ATM kinase. DMAP1 is required for DNA-damage induced TIP60-p400-mediated histone acetylation, and deletion of DMAP1 impairs AMT function (5). DMAP1-DNMT1 may also methylate DNA at sites of DNA damage during homologous recombination, which results in gene silencing (6).

    Database Links

    UniProt ID: Q9NPF5


    Entrez-Gene Id: 55929


    Limited Uses

    Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

    Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

    For Research Use Only. Not For Use In Diagnostic Procedures.
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