Dopamine Transporter/DAT (E3U7R) Rabbit mAb #24029
- IF
Supporting Data
| REACTIVITY | M |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Rabbit IgG |
Application Key:
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- M-Mouse
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Immunofluorescence (Frozen) | 1:100 - 1:400 |
Storage
Protocol
Specificity / Sensitivity
Dopamine Transporter/DAT (E3U7R) Rabbit mAb recognizes endogenous levels of total dopamine transporter/DAT protein. Non-specific labeling of mouse colon may be observed by immunofluorescence.
Species Reactivity:
Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro26 of mouse dopamine transporter/DAT protein.
Background
Dopamine is a neurotransmitter that plays important roles in the brain, particularly in dopamine pathways that control the motivational component of reward-motivated behavior. These behavioral outputs are generated by the basal ganglia via its interaction with multiple brain areas that modulate sensorimotor, emotional, and cognitive information (1). The spatiotemporal dynamics of dopamine signaling are regulated by the dopamine transporter (DAT), a presynaptic transmembrane protein that drives the reuptake of dopamine released into the synaptic cleft. This process occurs in a sodium- and chloride-dependent manner, with DAT transporting two sodium cations down their concentration gradient, along with one chloride anion and the dopamine substrate. DAT is a member of the neurotransmitter sodium symporter (NSS) family, which also includes serotonin, norepinephrine, glycine, and γ-aminobutyric acid (GABA) transporters. The NSS family is part of the larger solute carrier 6 (SLC6) family of proteins, which in addition to neurotransmitters, also include transporters of amino acids, osmolytes, and energy metabolites (2-5). Mutations in the DAT gene, SLC6A3, have been linked to Parkinson’s disease (PD), infantile parkinsonism-dystonia (IPD), autism spectrum disorder (ASD), attention deficit hyperactivity disorder (ADHD), major depressive disorder (MDD), and bipolar disorder (BP) (5-8). In addition to its role in the brain, DAT is expressed by lymphocytes and monocytes in the periphery, where it modulates immune function. Altered expression levels of DAT in the periphery is also a suggested biomarker for some cancers (9-12).
- Beaulieu, J.M. and Gainetdinov, R.R. (2011) Pharmacol Rev 63, 182-217.
- Vaughan, R.A. and Foster, J.D. (2013) Trends Pharmacol Sci 34, 489-96.
- Borre, L. et al. (2014) J Biol Chem 289, 25764-73.
- Navratna, V. and Gouaux, E. (2019) Curr Opin Struct Biol 54, 161-170.
- Bröer, S. and Gether, U. (2012) Br J Pharmacol 167, 256-78.
- Schmidt, S.G. et al. (2022) Nat Commun 13, 2446.
- DiCarlo, G.E. et al. (2019) J Clin Invest 129, 3407-3419.
- Reith, M.E.A. et al. (2022) Mol Psychiatry 27, 1031-1046.
- Mackie, P. et al. (2018) Brain Behav Immun 70, 21-35.
- Campa, D. et al. (2007) Lung Cancer 56, 17-23.
- Liu, S. et al. (2021) Pathol Res Pract 221, 153446.
- Schrödter, S. et al. (2016) Mol Cancer 15, 10.
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