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Render Timestamp: 2024-11-21T12:53:24.894Z
Commit: 5c4accf06eb7154018ba3f54329c7590f97f534a
XML generation date: 2024-09-30 01:59:31.708
Product last modified at: 2024-10-24T20:00:09.250Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
  1. Products /
  2. Primary Antibodies /
  3. Dopamine Transporter/DAT (E3U7R) Rabbit mAb
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Dopamine Transporter/DAT (E3U7R) Rabbit mAb #24029

Filter:
  • IF

    Supporting Data

    REACTIVITY M
    SENSITIVITY Endogenous
    MW (kDa)
    Source/Isotype Rabbit IgG
    Application Key:
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • M-Mouse 

    Product Information

    Product Usage Information

    Application Dilution
    Immunofluorescence (Frozen) 1:100 - 1:400

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Dopamine Transporter/DAT (E3U7R) Rabbit mAb recognizes endogenous levels of total dopamine transporter/DAT protein. Non-specific labeling of mouse colon may be observed by immunofluorescence.

    Species Reactivity:

    Mouse

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro26 of mouse dopamine transporter/DAT protein.

    Background

    Dopamine is a neurotransmitter that plays important roles in the brain, particularly in dopamine pathways that control the motivational component of reward-motivated behavior. These behavioral outputs are generated by the basal ganglia via its interaction with multiple brain areas that modulate sensorimotor, emotional, and cognitive information (1). The spatiotemporal dynamics of dopamine signaling are regulated by the dopamine transporter (DAT), a presynaptic transmembrane protein that drives the reuptake of dopamine released into the synaptic cleft. This process occurs in a sodium- and chloride-dependent manner, with DAT transporting two sodium cations down their concentration gradient, along with one chloride anion and the dopamine substrate. DAT is a member of the neurotransmitter sodium symporter (NSS) family, which also includes serotonin, norepinephrine, glycine, and γ-aminobutyric acid (GABA) transporters. The NSS family is part of the larger solute carrier 6 (SLC6) family of proteins, which in addition to neurotransmitters, also include transporters of amino acids, osmolytes, and energy metabolites (2-5). Mutations in the DAT gene, SLC6A3, have been linked to Parkinson’s disease (PD), infantile parkinsonism-dystonia (IPD), autism spectrum disorder (ASD), attention deficit hyperactivity disorder (ADHD), major depressive disorder (MDD), and bipolar disorder (BP) (5-8). In addition to its role in the brain, DAT is expressed by lymphocytes and monocytes in the periphery, where it modulates immune function. Altered expression levels of DAT in the periphery is also a suggested biomarker for some cancers (9-12).

    Database Links

    UniProt ID: Q61327


    Entrez-Gene Id: 13162


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    For Research Use Only. Not For Use In Diagnostic Procedures.
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