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Render Timestamp: 2025-01-21T21:30:28.049Z
Commit: da7e4f2f0d1aed1f1f8e20e4e2ecab8f33cbd595
XML generation date: 2024-09-30 01:57:32.487
Product last modified at: 2025-01-01T09:03:03.689Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

DPF3/BAF45C (E7F7N) Rabbit mAb #82788

Filter:
  • WB
  • IP
Western Blotting Image 1: DPF3/BAF45C (E7F7N) Rabbit mAb
Western blot analysis of extracts from SK-N-MC, PC-3, and HCT 116 cells using DPF3/BAF45C (E7F7N) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). As expected, HCT 116 cells are low for DPF3/BAF45C expression.

To Purchase # 82788

Cat. # Size Qty. Price Ships
82788T 20 µl
$153 Jan 22
82788S 100 µl
$339 Jan 22

Supporting Data

REACTIVITY H R
SENSITIVITY Endogenous
MW (kDa) 45, 47
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
  • IP-Immunoprecipitation 
Species Cross-Reactivity Key:
  • H-Human 
  • R-Rat 

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:100

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

DPF3/BAF45C (E7F7N) Rabbit mAb recognizes endogenous levels of total DPF3/BAF45C protein. Based on sequence, this antibody should detect both DPF3a and DPF3b.

Species Reactivity:

Human, Rat

The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.

Species predicted to react based on 100% sequence homology:

Mouse

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu146 of human DPF3/BAF45C protein.

Background

The modulation of chromatin structure is an essential component in the regulation of transcriptional activation and repression. Modifications can be made by at least two evolutionarily conserved strategies, through the disruption of histone-DNA contacts by ATP-dependent chromatin remodelers, or by histone tail modifications including methylation and acetylation. One of the four classes of ATP-dependent histone remodelers is the SWI/SNF complex, the central catalytic subunit of which is Brg1 or the highly related protein hBRM (1). This SWI/SNF complex contains varying subunits but its association with either Brg1 or hBRM remains constant (1). SWI/SNF complexes have been shown to regulate gene activation, cell growth, the cell cycle, and differentiation (1). Brg1/hBRM have been shown to regulate transcription through enhancing transcriptional activation of glucocorticoid receptors (2). Although usually associated with transcriptional activation, Brg1/hBRM have also been found in complexes associated with transcriptional repression, including HDACs, Rb, and Tif1β (3-5). Brg1/hBRM plays a vital role in the regulation of gene transcription during early mammalian embryogenesis. In addition, Brg1/hBRM also plays a role as a tumor suppressor and Brg1 is mutated in several tumor cell lines (6-8). DPF3/BAF45C is a member of the SWI/SNF complex that binds to acetylated and methylated histone tails via its double PHD finger domains. There are two splice variants in human and mouse, DPF3a and DPF3b, which differ in their C-terminus as DPF3a contains a single truncated PHD finger. DPF3/BAF45C has been shown to be a key regulator in heart and muscle development, and its phosphorylation removes HEY transcriptional repressors to induce cardiac hypertrophy (9-11). DPF3 has also been implicated in brown adipogenesis and NF-κB signaling (12,13).
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