DR5 (E9D7D) Rabbit mAb #69400
- WB
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 40, 48 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Simple Western™ | 1:10 - 1:50 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
DR5 is a receptor for TRAIL, which has been shown to induce apoptosis in a variety of cell types and has been targeted for cancer therapy (1-5). Structurally, DR5 contains an amino-terminal leader cleavage site, followed by an extracellular region containing two cysteine-rich repeats, a central transmembrane domain, and a carboxy-terminal DD. DR5 is expressed in a wide variety of tissues and is a transcriptional target of p53 (6-8). It induces apoptosis through a FADD-dependent pathway. Deletion of DR5 leads to resistance in TRAIL-mediated apoptosis as well as an abrogated response to DNA-damaging stimuli (9). At least two isoforms of DR5 are produced by alternative splicing (10).
- Nagata, S. (1997) Cell 88, 355-65.
- Thorburn, A. (2004) Cell Signal 16, 139-44.
- Wiley, S.R. et al. (1995) Immunity 3, 673-82.
- Walczak, H. et al. (1997) EMBO J 16, 5386-97.
- Chaudhary, P.M. et al. (1997) Immunity 7, 821-30.
- MacFarlane, M. et al. (1997) J Biol Chem 272, 25417-20.
- Wu, G.S. et al. (2000) Adv Exp Med Biol 465, 143-51.
- Wu, G.S. et al. (1997) Nat Genet 17, 141-3.
- Finnberg, N. et al. (2005) Mol Cell Biol 25, 2000-13.
- Screaton, G.R. et al. (1997) Curr Biol 7, 693-6.
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