Render Target: STATIC
Render Timestamp: 2024-11-26T11:17:49.650Z
Commit: d79925545b26f8827f92d145dadc6f0527debdb1
XML generation date: 2024-04-05 20:23:16.163
Product last modified at: 2024-05-30T07:08:05.673Z
1% for the planet logo
PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77

DSG2 (10D2) Mouse mAb (IF Specific) #12631

Filter:
  • IF

Inquiry Info. # 12631

Please see our recommended alternatives.

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 142
    Source/Isotype Mouse IgG1
    Application Key:
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Immunofluorescence (Immunocytochemistry) 1:100

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    DSG2 (10D2) Mouse mAb (IF Specific) recognizes endogenous levels of total DSG2 protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a recombinant protein specific to the amino terminus of human DSG2 protein.

    Background

    Desmosomes are a class of intracellular junction that tightly link adjacent cells in mechanically stressed tissues such as the epithelium and myocardium (1). They derive their characteristic strength from the protein desmoplakin, which acts as a tether by binding the cytoplasmic component of the desmosome at its N terminus (2), while its C terminus is anchored to the intermediate-filament cytoskeleton (3). Desmogleins and desmocollins belong to the superfamily of cadherin proteins, the "glue" of the desmosome, as they are essential for strong cell-cell contacts (4). There are 4 types of desmogleins in humans, DSG1-4, and 3 types of desmocollins, DSC1-3. DSG2 is expressed in all desmosome bearing tissues, while other desmosome cadherin proteins have more specialized tissue expression. Research studies have shown that aberrant expression due to mutation of DSG2 is associated with arrhythmogenic right ventricular cardiomyopathy (5,6). Research has also shown that mutation and altered expression of DSG1 and 3 have been associated with autoimmune disorders such as Pemphigus, inherited disorders such as defective hair-follicle differentiation, and striate palmoplantar keratoderma, an epidermal-thickening disease (reviewed in 7).
    For Research Use Only. Not For Use In Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    All other trademarks are the property of their respective owners. Visit our Trademark Information page.