Render Target: STATIC
Render Timestamp: 2024-10-11T10:38:10.521Z
Commit: 56767fe525c928647c8401233a175d0d607d385d
XML generation date: 2024-04-24 09:11:02.142
Product last modified at: 2024-09-25T22:00:08.272Z
View Featured Offers >>
1% for the planet logo
PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
  1. Products /
  2. Primary Antibodies /
  3. dsRNA (J2) Mouse mAb

dsRNA (J2) Mouse mAb #76651

Filter:
  • IF

    Supporting Data

    REACTIVITY All
    SENSITIVITY Endogenous
    MW (kDa)
    Source/Isotype Mouse IgG2a kappa
    Application Key:
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • All-All Species Expected 

    Product Information

    Product Usage Information

    Application Dilution
    Immunofluorescence (Immunocytochemistry) 1:500 - 1:1000

    Storage

    Supplied in a PBS solution that does not contain any stabilizers or preservatives and has been lyophilized. The lyophilized antibody is stable for 24 months when stored at -20°C. Reconstitute using 200 μL of sterile distilled water to create a 1 mg/mL solution. A slight precipitate may be present, but will not interfere with antibody performance. Recommended to centrifuge the reconstituted antibody and use the supernatant. Once reconstituted, this product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles.

    Protocol

    Specificity / Sensitivity

    dsRNA (J2) Mouse mAb recognizes dsRNA where the length of the helix is greater than or equal to 40 base pairs (bp). The ability to recognize dsRNA is independent of the sequence and nucleotide composition of the antigen. The J2 clone shows a higher affinity to natural dsRNA than to other dsRNA antigens (6).

    Species Reactivity:

    All Species Expected

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a nucleic acid sequence.

    Background

    Double-stranded RNA (dsRNA) is produced during the replication cycle of a broad range of viruses and can trigger the innate immune response. dsRNA is present if cells are infected with a dsRNA virus or it can be generated during the course of single-stranded RNA (ssRNA) virus replication. Host cells do not produce dsRNA so the innate immune response can differentiate between host and viral RNAs by the presence of dsRNA (1). The innate immune response is controlled by several classes of germline-encoded pattern-recognition receptors (PRRs), including retinoic acid-inducible gene-I (RIG-I)-like receptors (RLRs), Toll-like receptors (TLRs), and NOD-like receptors (NLRs) that sense viral components, such as dsRNA, ssRNA, and DNA (2,3). Activation of PRRs by dsRNA leads to production of type I interferons (IFNs) and proinflammatory cytokines, triggering the adaptive immune response (2,3). Understanding how antiviral responses are initiated, the counter strategies that viruses adopt, and exploring the viral life cycle gives researchers a better comprehension of how to treat infections of different viral diseases (4,5).

    Limited Uses

    Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

    Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

    For Research Use Only. Not For Use In Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    This product is a SCICONS brand antibody manufactured by Absolute Biotech.
    All other trademarks are the property of their respective owners. Visit our Trademark Information page.