Render Target: STATIC
Render Timestamp: 2024-11-22T11:07:43.687Z
Commit: 5c4accf06eb7154018ba3f54329c7590f97f534a
XML generation date: 2024-08-01 15:29:58.713
Product last modified at: 2024-11-06T13:30:34.071Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

DUSP3/VHR Antibody #4752

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H M R Mk
    SENSITIVITY Endogenous
    MW (kDa) 20
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    DUSP3/VHR Antibody detects endogenous levels of total DUSP3 protein. This antibody does not cross-react with other dual-specific phosphatases.

    Species Reactivity:

    Human, Mouse, Rat, Monkey

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to human DUSP3. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    DUSP3, also known as VHR (VH1 related) is a small dual-specific phosphatase with specificity for MAP kinase ERK1/2 and JNK, but not for p38 MAPK (1,2). Unlike most of the dual-specific phosphatases, which have inducible expression patterns, DUSP3 is constitutively expressed (2). In antigen stimulated T cells, DUSP3 is phosphorylated by ZAP-70 at Tyr138 (3). Tyr138 phosphorylation is required for DUSP3 to down-regulate the ERK and JNK pathways (3).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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