DUSP3/VHR Antibody #4752
- WB
- IP
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 20 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Protocol
Specificity / Sensitivity
DUSP3/VHR Antibody detects endogenous levels of total DUSP3 protein. This antibody does not cross-react with other dual-specific phosphatases.
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to human DUSP3. Antibodies are purified by protein A and peptide affinity chromatography.
Background
DUSP3, also known as VHR (VH1 related) is a small dual-specific phosphatase with specificity for MAP kinase ERK1/2 and JNK, but not for p38 MAPK (1,2). Unlike most of the dual-specific phosphatases, which have inducible expression patterns, DUSP3 is constitutively expressed (2). In antigen stimulated T cells, DUSP3 is phosphorylated by ZAP-70 at Tyr138 (3). Tyr138 phosphorylation is required for DUSP3 to down-regulate the ERK and JNK pathways (3).
Limited Uses
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