Render Target: STATIC
Render Timestamp: 2024-11-22T11:10:22.589Z
Commit: 5c4accf06eb7154018ba3f54329c7590f97f534a
XML generation date: 2024-09-30 01:54:04.670
Product last modified at: 2024-11-18T12:45:32.270Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

E4BP4/NFIL3 (D5K8O) Rabbit mAb #14312

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 60
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:100

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    E4BP4/NFIL3 (D5K8O) Rabbit mAb recognizes endogenous levels of total E4BP4/NFIL3 protein.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro455 of human E4BP4/NFIL3 protein.

    Background

    E4BP4/NFIL3 is a basic leucine zipper transcriptional regulator that plays a variety of roles in the immune system (1). For example, E4BP4/NFIL3 is required for bone marrow-derived NK cell development (2,3). In addition, it regulates IgE class switching in B cells by controlling IL-4 dependent induction of germ-line ε (GLε) transcription (4). In macrophages, E4BP4/NFIL3 is induced following exposure to bacteria and negatively regulates transcription of IL-12 p40. E4BP4/NFIL3 also controls cytokine production by T cells. Th2 cells from E4BP4/NFIL3(-/-) mice produced elevated levels of IL-5 an IL-13, but reduced levels of IL-4 ad IL-10 (5,6). Chronically stimulated Th1 cells, regulatory T cells, and NKT cells from E4BP4/NFIL3(-/-) mice all produce reduced levels of IL-10 and IL-13 (6). Finally, E4BP4/NFIL3 is also required for the development of conventional CD8α+ dendritic cells, as this subset is absent from E4BP4/NFIL3(-/-) mice (7).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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