EAAT1 (D20D5) Rabbit mAb #5685
- WB
- IP
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 58 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
EAAT2 accounts for up to 90% of the total glutamate transport in brain while EAAT1 contributes the remaining 5-10% (4). The contribution of EAAT1 in neurotransmission is unclear since EAAT2 is much more abundant. However, EAAT1 expression is upregulated by increasing concentrations of glutamate in the media of cultured primary astrocytes, potentially giving this glutamate transporter additional importance (5). EAAT1 has neuroprotective potential following ischemia since reactive astrocytes and activated microglia express EAAT1 but not EAAT2 (6).
- Amara, S.G. and Fontana, A.C. (2002) Neurochem Int 41, 313-8.
- Tanaka, K. et al. (1997) Science 276, 1699-702.
- Casado, M. et al. (1993) J Biol Chem 268, 27313-7.
- Hediger, M.A. (1999) Am J Physiol 277, F487-92.
- Gegelashvili, G. et al. (1996) Neuroreport 8, 261-5.
- Beschorner, R. et al. (2007) Histopathology 50, 897-910.
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