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Render Timestamp: 2024-11-21T14:14:01.554Z
Commit: 5c4accf06eb7154018ba3f54329c7590f97f534a
XML generation date: 2024-09-30 01:58:34.197
Product last modified at: 2024-09-30T08:01:02.573Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
  1. Products /
  2. Primary Antibodies /
  3. EAAT4 (E6E9I) Rabbit mAb
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

EAAT4 (E6E9I) Rabbit mAb #69908

Filter:
  • WB
  • IF

    Supporting Data

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 20, 22, 50, 70, 80
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunofluorescence (Frozen) 1:200 - 1:400

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    EAAT4 (E6E9I) Rabbit mAb recognizes endogenous levels of total EAAT4 protein.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly552 of human EAAT4 protein.

    Background

    During neurotransmission, glutamate is released from vesicles of the presynaptic cell, and glutamate receptors (e.g., NMDA receptor, AMPA receptor) bind glutamate for activation at the opposing postsynaptic cell. Excitatory amino acid transporters (EAATs) regulate and maintain extracellular glutamate concentrations below excitotoxic levels (1,2). In addition, glutamate transporters may limit the duration of synaptic excitation by an electrogenic process in which the transmitter is cotransported with three sodium ions and one proton, followed by countertransport of a potassium ion (1,2). Five EAATs (EAAT1-5) have been identified. EAAT1 and EAAT2 are expressed mainly in glia, while EAAT3, EAAT4, and EAAT5 are considered to be neuronal transporters (2). EAAT4 (encoded by the SLC1A6 gene) is a neuronal glutamate transporter expressed in Purkinje cells of the cerebellum (3). EAAT4 plays an important role in maintaining neuronal synaptic function, particularly in the cerebellum, by maintaining appropriate extracellular glutamate levels to prevent neurotoxicity and permit effective synaptic communication. EAAT4 function may be regulated by post-translational modification (4,5). EAAT4 may be a therapeutic target to treat several neurological diseases, including spinocerebellar ataxias, by restoring extracellular glutamate homeostasis (6). 

    Database Links

    UniProt ID: P48664


    Entrez-Gene Id: 6511


    Limited Uses

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    For Research Use Only. Not For Use In Diagnostic Procedures.
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