Render Target: STATIC
Render Timestamp: 2024-12-20T10:51:51.539Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-09-30 01:58:37.006
Product last modified at: 2024-12-17T19:02:20.137Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
  1. Products /
  2. Primary Antibodies /
  3. ECM1 (E3B9A) Rabbit mAb
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

ECM1 (E3B9A) Rabbit mAb #83458

Filter:
  • WB
  • IF

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 75-100
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunofluorescence (Immunocytochemistry) 1:800 - 1:3200

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    ECM1 (E3B9A) Rabbit mAb recognizes endogenous levels of total ECM1 protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human ECM1 protein.

    Background

    Extracellular matrix gene 1 (ECM1) is a secreted glycoprotein found primarily in the extracellular matrix. It acts as both a multifunctional binding core and as a scaffolding protein that interacts with a variety of extracellular and structural proteins, including perlecan, fibulin-1C/1D, and MMP-9. (1). Research studies have shown that ECM1 is involved in the maintenance of tissue integrity and homeostasis, T cell immune responses, and angiogenesis (2-5). Its importance in maintaining tissue integrity is demonstrated by reports showing that mutations in the gene encoding ECM1 lead to skin lipoid proteinosis (1). Notably, ECM1 expression is upregulated in a variety of cancers, and during metastasis, suggesting a role for ECM1 in promoting tumor cell proliferation and invasion (6). In this context, research studies have shown that ECM1 can activate various signaling pathways, such as integrin/FAK, MMP-9/galectin-3, and S100A4/RhoA (7-9). In hepatic tissues, it was furthermore shown that ECM1 contributes to maintaining the latency of TGF-β in the extracellular matrix, thereby preventing spontaneous TGF-β activation and liver fibrosis (10,11).

    Database Links

    UniProt ID: Q16610


    Entrez-Gene Id: 1893


    Limited Uses

    Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

    Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

    For Research Use Only. Not For Use In Diagnostic Procedures.
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