eIF3J Antibody #3261
- WB
- IP
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 35 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
One of the smallest subunits of eIF3, eIF3J, is critical in 40S initiation complex assembly (5). eIF3J has been shown to associate with the aminoacyl site and mRNA entry channel of the 40S ribosomal subunit (6). eIF3J has also been shown to have an additional role in the recycling of post-termination complexes (7).
- Masutani, M. et al. (2007) EMBO J 26, 3373-83.
- Chaudhuri, J. et al. (1999) J Biol Chem 274, 17975-80.
- Holz, M.K. et al. (2005) Cell 123, 569-80.
- Harris, T.E. et al. (2006) EMBO J 25, 1659-68.
- Fraser, C.S. et al. (2004) J Biol Chem 279, 8946-56.
- Fraser, C.S. et al. (2007) Mol Cell 26, 811-9.
- Pisarev, A.V. et al. (2007) Cell 131, 286-99.
Limited Uses
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