Render Target: STATIC
Render Timestamp: 2024-11-22T10:38:21.077Z
Commit: 5c4accf06eb7154018ba3f54329c7590f97f534a
XML generation date: 2024-08-30 10:37:39.064
Product last modified at: 2024-08-31T07:01:33.964Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77

eNOS (6H2) Mouse mAb (BSA and Azide Free) #26339

Filter:
  • WB
  • IHC

    Supporting Data

    REACTIVITY H B
    SENSITIVITY Endogenous
    MW (kDa) 140
    Source/Isotype Mouse IgG1
    Application Key:
    • WB-Western Blotting 
    • IHC-Immunohistochemistry 
    Species Cross-Reactivity Key:
    • H-Human 
    • B-Bovine 

    Product Information

    Product Usage Information

    This product is the carrier free version of product #5880. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

    This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.

    BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.

    Formulation

    Supplied in 1X PBS (10 mM Na2HPO4, 3 mM KCl, 2 mM KH2PO4, and 140 mM NaCl (pH 7.8)). BSA and Azide Free.

    For standard formulation of this product see product #5880

    Storage

    Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

    Specificity / Sensitivity

    eNOS (6H2) Mouse mAb (BSA and Azide Free) recognizes endogenous levels of total eNOS protein.

    Species Reactivity:

    Human, Bovine

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with recombinant protein specific to human eNOS protein.

    Background

    Endothelial nitric-oxide synthase (eNOS) is an important enzyme in the cardiovascular system. It catalyzes the production of nitric oxide (NO), a key regulator of blood pressure, vascular remodeling, and angiogenesis (1,2). The activity of eNOS is regulated by phosphorylation at multiple sites. The two most thoroughly studied sites are the activation site Ser1177 and the inhibitory site Thr495 (3). Several protein kinases including Akt/PKB, PKA, and AMPK activate eNOS by phosphorylating Ser1177 in response to various stimuli (4,5). In contrast, bradykinin and H2O2 activate eNOS activity by promoting both Ser1177 phosphorylation and Thr495 dephosphorylation (6,7).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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