Render Target: STATIC
Render Timestamp: 2024-11-20T11:35:12.064Z
Commit: 5c4accf06eb7154018ba3f54329c7590f97f534a
XML generation date: 2024-09-30 01:56:04.001
Product last modified at: 2024-11-18T21:45:09.407Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77

eNOS (6H2) Mouse mAb #5880

Filter:
  • WB
  • IHC

    Supporting Data

    REACTIVITY H B
    SENSITIVITY Endogenous
    MW (kDa) 140
    Source/Isotype Mouse IgG1
    Application Key:
    • WB-Western Blotting 
    • IHC-Immunohistochemistry 
    Species Cross-Reactivity Key:
    • H-Human 
    • B-Bovine 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunohistochemistry (Paraffin) 1:200

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    For a carrier free (BSA and azide free) version of this product see product #26339.

    Protocol

    Specificity / Sensitivity

    eNOS (6H2) Mouse mAb recognizes endogenous levels of total eNOS protein.

    Species Reactivity:

    Human, Bovine

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with recombinant protein specific to human eNOS protein.

    Background

    Endothelial nitric-oxide synthase (eNOS) is an important enzyme in the cardiovascular system. It catalyzes the production of nitric oxide (NO), a key regulator of blood pressure, vascular remodeling, and angiogenesis (1,2). The activity of eNOS is regulated by phosphorylation at multiple sites. The two most thoroughly studied sites are the activation site Ser1177 and the inhibitory site Thr495 (3). Several protein kinases including Akt/PKB, PKA, and AMPK activate eNOS by phosphorylating Ser1177 in response to various stimuli (4,5). In contrast, bradykinin and H2O2 activate eNOS activity by promoting both Ser1177 phosphorylation and Thr495 dephosphorylation (6,7).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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