Cell Signaling Technology Logo

Basket Updated

0

Items added

1% for the planet logo
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

eNOS (D8A6N) Rabbit mAb #35362

Filter:
  • WB
  • IHC
Western Blotting Image 1: eNOS (D8A6N) Rabbit mAb
Western blot analysis of extracts from EA.hy.926, HeLa and HUVEC cells using eNOS (D8A6N) Rabbit mAb (upper) or β-Tubulin (D2N5G) Rabbit mAb #15115 (lower).

To Purchase # 35362

Cat. # Size Qty. Price
35362T 20 µl
$153
35362S 100 µl
$339

Supporting Data

REACTIVITY H B
SENSITIVITY Endogenous
MW (kDa) 140
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
  • IHC-Immunohistochemistry 
Species Cross-Reactivity Key:
  • H-Human 
  • B-Bovine 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunohistochemistry (Paraffin) 1:100

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

eNOS (D8A6N) Rabbit mAb recognizes endogenous levels of total eNOS protein. This antibody does not cross-react with iNOS or nNOS proteins.

Species Reactivity:

Human, Bovine

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro1190 of human eNOS protein.

Background

Endothelial nitric-oxide synthase (eNOS) is an important enzyme in the cardiovascular system. It catalyzes the production of nitric oxide (NO), a key regulator of blood pressure, vascular remodeling, and angiogenesis (1,2). The activity of eNOS is regulated by phosphorylation at multiple sites. The two most thoroughly studied sites are the activation site Ser1177 and the inhibitory site Thr495 (3). Several protein kinases including Akt/PKB, PKA, and AMPK activate eNOS by phosphorylating Ser1177 in response to various stimuli (4,5). In contrast, bradykinin and H2O2 activate eNOS activity by promoting both Ser1177 phosphorylation and Thr495 dephosphorylation (6,7).

Pathways

Explore pathways related to this product.


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
SignalStain is a registered trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit our Trademark Information page.