eNOS (D9A5L) Rabbit mAb (BSA and Azide Free) #86374
- WB
- IF
Supporting Data
| REACTIVITY | H M R B |
| SENSITIVITY | Endogenous |
| MW (kDa) | 140 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- B-Bovine
Product Information
Product Usage Information
This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.
Formulation
Storage
Specificity / Sensitivity
eNOS (D9A5L) Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total eNOS protein. This antibody does not cross-react with overexpressed iNOS nor nNOS.
Species Reactivity:
Human, Mouse, Rat, Bovine
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro1190 of human eNOS protein.
Background
Endothelial nitric-oxide synthase (eNOS) is an important enzyme in the cardiovascular system. It catalyzes the production of nitric oxide (NO), a key regulator of blood pressure, vascular remodeling, and angiogenesis (1,2). The activity of eNOS is regulated by phosphorylation at multiple sites. The two most thoroughly studied sites are the activation site Ser1177 and the inhibitory site Thr495 (3). Several protein kinases including Akt/PKB, PKA, and AMPK activate eNOS by phosphorylating Ser1177 in response to various stimuli (4,5). In contrast, bradykinin and H2O2 activate eNOS activity by promoting both Ser1177 phosphorylation and Thr495 dephosphorylation (6,7).
- Fulton, D. et al. (2001) J Pharmacol Exp Ther 299, 818-24.
- Shaul, P.W. (2002) Annu Rev Physiol 64, 749-74.
- Chen, Z.P. et al. (1999) FEBS Lett 443, 285-9.
- Dimmeler, S. et al. (1999) Nature 399, 601-5.
- Fulton, D. et al. (1999) Nature 399, 597-601.
- Harris, M.B. et al. (2001) J Biol Chem 276, 16587-91.
- Thomas, S.R. et al. (2002) J Biol Chem 277, 6017-24.
Limited Uses
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